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           Manipal Center for Infectious Diseases

Prasanna School of Public Health
Manipal Academy of Higher Education

Seed Grant Award


Terms of reference for Seed Grant Award 2021-2022

MAC ID Seed Grant Awardees 2021-2022

Dr Shwethapriya Rao - Professor & Head of Critical Care Medicine, KMC Manipal

Dr Apurv Barche – Assistant Professor of Pediatrics, KMC Manipal

Archana Mahadev Rao - Assistant Professor of Biotechnology, MIT Manipal

Dr Mridula M - Associate Professor of Microbiology, KMC Manipal

Dr Ciraj Ali Mohammed - Professor of Microbiology, MMMC Manipal

Dr Shaik Mahammad Abdul Fayaz - Assistant Professor of Biotechnology, MIT Manipal

MAC ID Seed Grant Awardees 2020-2021
Dr Nitin Gupta - Assistant professor of Infectious Diseases, KMC Manipal

Title of Seed grant project: "Impact of Rapid diagnostic tests in patients with acute febrile illness presenting to a tertiary care hospital in South India: an open diagnostic intervention randomized controlled trial"
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Dr Bharath Raja Guru - Professor of Biotechnology, MIT Manipal

Title of Seed grant project: Implant and nano-formulation of acyclovir and prodrug of acyclovir using biodegradable polymers to treat herpes simplex virus infections
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Dr Kanav Khera - Assistant Professor of Pharmacy Practice, MCOPS Manipal

Title of Seed grant project: Genotypic profiling and docking studies using gyrA gene obtained from Fluoroquinolone-resistant urinary isolates of E. coli
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Dr Suchitra Shenoy - Associate Professor of Microbiology, KMC Mangalore

Title of Seed grant project: Nanoparticle - encapsulated antibiotics to combat bacterial biofilm
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Dr Barnini Banerjee - Associate Professor of Microbiology, KMC Manipal

Title of Seed grant project: Molecular epidemiology of the methicillin resistant Staphylococcus aureus and their association with biofilm formation in patients with bone and joint infection
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MAC ID Seed Grant Awardees 2019-20
Dr Dhanashree B - Associate Professor of Microbiology, KMC Mangalore

Title of Seed grant project: Detection of common diarrheagenic bacterial and viral pathogens in faecal samples of paediatric by polymersae cahin reaction
Background: Diarrheal diseases are the primary cause of death among children below five years of age. Aetiology of diarrhoea differs depending on the country and region. Most of the clinical laboratories perform microscopy and culture which have reduced sensitivity and hence aetiology of diarrhoea goes undiagnosed. Accurate laboratory diagnosis of the diarrhoea causing agent is necessary to know the pathogen-specific burden of diarrhoeal disease and management of patients.

Material & methods: In this cross-sectional, in-vitro study done from 01-April-2019 to 31-March-2020 in the Department of Microbiology, Kasturba Medical College, Mangalore, stool samples of children (n=109) with ≥3 diarrheal episodes were cultured for bacterial pathogens. Total faecal genomic DNA & RNA was extracted using QIAamp Fast DNA Stool and QIAamp Viral RNA Mini Kits. Real time PCR (RT PCR) was done for the detection of bacterial and viral aetiological agents.

Results: Among the 109 stool samples screened by RT PCR, Shigella spp was detected in 16% (n=17), Rotavirus in 21% (n=23) and Salmonella spp. in 2% (n=2) of stool samples. Two samples had multiple pathogens namely Rotavirus and Shigella spp. However, by the conventional culture, we could isolate one each of Shigella flexneri & S.Typhi from two samples.

Conclusion: Shigella spp and Rotavirus were the predominant aetiology of diarrhoea in our study population. PCR was found to be a better tool when compared to culture as it could detect both viral and bacterial pathogens simultaneously. Further studies are necessary to screen for all the possible pathogens (bacteria, virus & parasite) in stool samples with a focus on the seasonal distribution of the pathogens causing diarrhoea in this part of the country.
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Dr Sridevi HB - Associate Professor of Pathology, KMC Mangalore

Title of Seed grant project: Utility of serum procalcitonin and CD64 ratio expressed on neutrophill, lymphocyte and monocyte subsets in diagnosis of early Onset Neonatal Sepsis
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Dr Chythra Rao - Associate Professor, Dept. of Community Medicine, KMC Manipal

Title of Seed grant project:Seasonal trends in microbial quality of community water samples and perpectives about antibiotic use and misuse in the community
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Title of Project Agency Grant Amount
Socio-cultural and environmental drivers of antibiotic resistance in the community: people, physicians and pharmacists’ perspective – a mixed methods approach. ICMR INR 27,20,933/-

Dr Sonal Sekhar - Assistant Professor, Dept. of Pharmacy Practice, MCOPS, Manipal

Title of Seed grant project: Vitamin D Supplements in Diabetic Foot Infection: A Prospective Observational Study in a Tertiary Healthcare Facility
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Dr Jesil Mathew A - Associate Professor, Dept of Pharmaceutical Biotechnology, MCOPS, Manipal

Title of Seed grant project: Design and Evaluation of a Nanogel formulation for Bioflim based burn Infections
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Dr Shashidhar V - Associate Professor of Microbiology, KMC Manipal

Title of Seed grant project: Screening for Abacavir Hypersensitivity by DNA Microarray among HIV sero-positive adult patients in a tertiary care hospital
HLA molecules are involved in induction and regulation of immune responses, but are associated with disease too. HLA-B*5701 allele carriage has been strongly associated with hypersensitivity reaction to abacavir (ABC) with up to 100% positive predictive value. Occurrence (<5%) of hypersensitivity reaction (HSR) has restricted the usage of ABC. A prospective study was conducted to assess the frequency of HLAB*57:01 allele carriage by DNA Microarray among 52 HIV-1 infected individuals attending HIV clinic and to document the usage of ABC and its adverse event profile. The mean age of the study subjects was 49 yrs, with majority of them males (67%). Antiretroviral therapy was started in 90% patients. The most frequent ART regimen (60%) instituted was a combination of 2 NRTIs and 1 NNRTI. Mean CD4+ lymphocyte cell count was 480 cells/cu.mm. HIV-1 viral load of less than 1000 copies/mL was found in 67% study subjects. HLA-B*57:01 carriage was seen in 2 (3.8%) patients who were not on ABC based ART regimen currently or in the past. Even though low sample size was a limitation, the frequency rate which we found reflects the prevalence rates found in earlier studies (ASSURE) including those from India.

The frequency of ABC usage in our hospital setting was found to be low. During the study period, 10 patients were found to be on ABC based ART regimen, including 6 patients for whom HLAB*57:01 testing was performed. Tenofovir induced renal adverse effects (50%), chronic kidney disease (30%) and hepatitis with dolutegravir based regimen (20%) were the reasons for introduction of ABC. None of the patients on ABC had experienced signs or symptoms suggestive of ABC-associated HSR.

Predicting the occurrence of ABC HSR or its avoidance through HLA-B*57:01 screening is possible. Considering the evidence of varied prevalence of HLAB*57:01 across different racial and ethnic populations and gender wise differences in allele carriage, it is appropriate that multi-centric studies across India may be planned to know the HLAB*57:01 prevalence rates across different geographical regions of our country among HIV-infected individuals. Cost can be a limiting factor for HLAB*57:01 screening which needs consideration. Kit based microarray testing seems to require relatively less technical expertise and also allows easy analysis of results compared to other molecular typing methods.

Screening for HLA-B*5701 allele carriage among HIV-infected patients should be performed before initiation of therapy with ABC-containing regimens. There is further scope to work on the role of various HLA phenotypes and clinical outcomes in HIV infection in Indian settings.
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MAC ID Seed Grant Awardees 2017-18
Dr G Sreejith - Associate Professor, Dept. of Microbiology, MMMC, Manipal

Title of Seed grant project: Developing and evaluating the efficacy of a paper based rapid diagnostic test for dengue using zinc oxide quantum dots
Integration of medicine with technology is the solution for efficient and accelerated diagnostic outcomes to deliver better health care. Efforts are being taken by many researchers to develop economical and easy to use diagnostic assays for various clinical conditions including infectious diseases. Introduction of nanotechnology has been noticed as a major breakthrough in many such successful research initiatives. Usage of nanobiosensors are shown to be an excellent approach for a better diagnostic test. The objective of this project was to develop a paper-based dengue detection kit. In view of that, luminescent ZnO nanoparticles functionalized with specific anti NS1 antibody was synthesized. Luminescence spectra of the ZnO nanoparticles showed two peaks at 370 nm and 440 nm. The changes in the luminescence intensity with different amount of dengue positive serum was studied and compared with changes in the nanoparticle luminescence intensity with different amount dengue negative serum samples. It was observed that, after adding positive serum, luminescence intensity of the ZnO nanoparticles increased, which can be attributed to the binding of the NS1 antigen to the antibody. After adding negative serum, the luminescence intensity of the ZnO nanoparticles showed a decrease in intensity. This confirmed the possibility of using changes in the luminescence of ZnO nanoparticles for dengue detection. Further optimization and studies are required to understand these changes in the luminescence and to develop a dengue detection kit.

   

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Dr Sindhura Lakshmi - Assistant Professor, Dept. of Pathology, KMC, Manipal

Title of Seed grant project: Role of intracellular cytokine flow cytometry in assessing tuberculosis specific cytokines to differentiate between active and latent tuberculosis
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Dr Naresh Kumar Mani - Associate Professor, Dept. of Biotechnology, MIT, Manipal

Title of Seed grant project:PADS: Engineering Miniaturized Paper-based Analytical Devices for Point-of-Care (POC) diagnosis & antibiotic susceptibility of Candidiasis
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Anusha Prabhu, Giri Nandagopal M. S., Prakash Peralam Yegneswaran, Vijendra Prabhu, Ujjwal Verma, Naresh Kumar Mani; Thread integrated smart-phone imaging facilitates early turning point colorimetric assay for microbes; RSC Adv., 2020, 10, 26853; DOI: 10.1039/d0ra05190j.
Quartile 1; Impact factor – 3.1
Publication Link: https://pubs.rsc.org/en/Content/ArticleLanding/2020/RA/D0RA05190J#!divAbstract

Prabhu, A., Giri Nandagopal, M.S., Peralam Yegneswaran, P. et al. Inkjet printing of paraffin on paper allows low-cost point-of-care diagnostics for pathogenic fungi. Cellulose (2020).
Quartile 1; Impact factor – 4.2
Publication Link: https://doi.org/10.1007/s10570-020-03314-3

Patent filed: A Paraffin Based Composition and Paper Based System for Detection of Microbes

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Dr Sevitha Bhat - Associate Professor, Dept. of Microbiology, KMC, Mangalore

Title of Seed grant project: Utility of direct detection of mec A gene in clinical specimen for detection of Methicillin Resistant Staphylococcus aureus.
Background: S. aureus is implicated in skin, soft tissue infections and invasive infections like cellulitis pneumonia, endocarditis, bacteremia, septic shock. MRSA infections are associated with higher morbidity, mortality and higher costs MRSA: major cause of heath care associated infections (HA -MRSA) HA-MRSA rates have increased from 23.9% in 2013 to 30.2% in 2016 The alarming rise in the rates of HA- MRSA reported from this region necessitates the need for a DNA based assay PCR: easy, sensitive and specific method for detection of MRSA .

Objectives: To isolate and identify MRSA from clinical specimen , To detect mec A gene in the clinical specimen and To compare direct detection of mec A gene with detection in clinical isolate of Staphylococcus aureus

Methods: Cross sectional hospital based study Inclusion criteria: Invasive samples (blood,deep tissue pus, bronchoalveolar lavage, sterile body fluids )Sample size: 125. The samples were processed using standard methods. Identification of growth: Gram stain, catalase test &coagulase test. Antibiotic sensitivity testing: Modified Kirby-Bauer disk diffusion method. The results were interpreted with CLSI recommendations. Cefoxitin (30μg) disk diffusion method: methicillin resistance in S.aureus. DNA Extraction: QIAamp blood and tissue kit (QIAGEN) and alkali lysis for the extraction from BacT alert broth and Real time PCR was performed.

Results: 110 clinical non duplicate samples were included in the study The prevalence of MRSA : 29%.74.6% : skin and soft tissue infections,11% from blood stream infections , 10.4% from osteomyelitis cases, 4% respiratory secretions Antibiotic resistance rates of MRSA ciprofloxacin(75%),clindamycin(51.1%), trimethoprim sulfamethoxazole (59.1%) and erythromycin( 62.5%). Vancomycin (0%). Real time PCR Sensitivity: 97% Specificity: 98% PPV:94% ,NPV : 98% (With culture as gold standard )

Conclusion: This study demonstrated the potential utility of real-time PCR for the detection of S. aureus and MRSA directly from clinical cultures allowing for rapid same day detection. We used Gram stain results as criteria for sample selection. Rapid detection of MRSA would assist in better patient management, in terms of accurate antimicrobial treatment, help in cohorting infected from uninfected patient to prevent spread.
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Dr Navya Vyas - Assistant Professor, Dept of Public Health, PSPH, Manipal

Title of Seed grant project: Multicentric Observational Study on Protective Effect of Metformin against Tuberculosis in Diabetic Patients in South Indian Tertiary Healthcare Facilities
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Dr Ritu Raval - Associate Professor, Dept. of Biotechnology, MIT, Manipal

Title of Seed grant project:Exploring the immunomodulatory properties of chitosan derivatives
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Mudgal J, Mudgal PP, Kinra M, Raval R. Immunomodulatory role of chitosan‐based nanoparticles and oligosaccharides in cyclophosphamide‐treated mice. Scand J Immunol. 2019;e12749
Quartile 3; Impact factor – 2.314
Publication Link: https://onlinelibrary.wiley.com/doi/full/10.1111/sji.12749


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MAC ID Seed Grant Awardees 2016-17
Dr Kavitha Saravu - Professor & Head, Dept. of Infectious Diseases, KMC Manipal

Title of Seed grant project: Comparative Therapeutic Efficacy Study of Two Regimens of Primaquine for Radical Cure of Plasmodium Vivax Malaria among Adults in Udupi
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A pilot randomized controlled trial to compare the effectiveness of two 14-day primaquine regimens for the radical cure of vivax malaria in South India. 2018; Mal J, 17, 321.
Quartile 1; Impact factor - 2.845
Publication Link: https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2472-5

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Title of Project Agency Grant Amount
Gazelle Malaria Clinical Accuracy study of a novel point of care test Hemex Health, USA INR 7,69, 537/-

Dr Chiranjay Mukhopadhyay - Associate Dean, KMC Manipal & Professor Dept. Microbiology, KMC Manipal

Title of Seed grant project: Molecular Detection and Characterization of Carbapenemase Resistance Gram Negative Bacteria (Cr-Gnb) among Patients with Sepsis
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Dr T S Murali - Associate Professor, Dept. of Biotechnology, Manipal School of Life Sciences, Manipal

Title of Seed grant project:Microbial synergy and altered virulence in polymicrobial biofilms
Diabetic foot ulcers (DFUs) are one of the most serious and long-term complications associated with diabetes with approximately 16% of the diabetic patients developing foot ulcers at least once in their life time (IDF, 2019). Infected DFU are commonly associated with complex polymicrobial communities known as biofilms. Biofilms typically comprise of extracellular polymeric matrix where clusters of bacteria remain adhered to an abiotic or biotic substrate. It is well documented that chronic wound infections are biofilm-mediated (James et al., 2008) and biofilm production facilitates the persistence of antibiotic resistant isolates. In addition to providing bacteria the environmental protection, nutritional requirements, and improved cell-to-cell communication, biofilms also provide bacteria with increased antibacterial resistance, and increased virulence (Costerton et al., 2003; Trøstrup et al., 2013) thereby predisposing them to delayed wound healing (Zhao et al., 2010). Treating biofilm infection caused by multidrug resistant bacteria is a major and daunting challenge considering that antibacterial drug development has not seen much success recently. Our study reports the biofilm forming capabilities of the dominant bacterial isolates obtained from DFUs along with their resistant profiles against various clinically relevant antibiotics. The study also looks into major virulence factors and pathogen–pathogen interaction at the genetic level in biofilm communities and strategies to combat multi drug resistance in these bacteria with the help of naturally occurring phytocompounds thereby aiding better management of DFUs in diabetic patients.
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Dr Shalini Shenoy - Professor, Dept. of Microbiology, KMC Mangalore

Title of Seed grant project:Clinicomicrobiological profile of Gram negative bacterial infections in Adult ICU’s of a tertiary care centre.
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Shalini Shenoy Mulki, Sevitha Bhat, Pooja Rao. Clinicomicrobiological Profile of Gram Negative Bacterial Infections in Adult ICU’s of a Tertiary Care Centre. Indian J Public Health Research & Development, 11(3), 326-331.
Quartile 4; Impact factor - 0.154
Publication Link: https://medicopublication.com/index.php/ijphrd/article/view/1142

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Dr Sneha Deepak Mallya - Associate Professor, Dept. of Community Medicine, KMC Manipal

Title of Seed grant project:A study to estimate the prevalence of metabolic syndrome, cardiovascular risk factors and its determinants among ART naïve and treated adult patients infected with HIV
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Sneha Deepak Mallya, Sravan Kumar Reddy T, Asha Kamath, Akhilesh Kumar Pandey, Kavitha Saravu, "Determinants of Metabolic Syndrome and 5-Year Cardiovascular Risk Estimates among HIV-Positive Individuals from an Indian Tertiary Care Hospital", AIDS Research and Treatment, vol. 2020. Quartile 2; Impact Factor: 0.882
Publication Link: https://doi.org/10.1155/2020/5019025

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Dr K.Vidyalakshmi - Professor and Head, Dept. of Microbiology, KMC, Mangalore

Title of Seed grant project:Association of Virulence Genes with Clinical Presentations and Outcomes in Melioidosis
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Dr Kiran Chawla - Professor, Dept. of Microbiology, KMC, Manipal

Title of Seed grant project:Detection of Mycobacterium tuberculosis specific host miRNA marker for diagnosis of extra Pulmonary tuberculosis
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Sanjiban Chakrabarty, Ajay Kumar, Kuthethur Raviprasad , Sandeep Mallya , Kapaettu Satyamoorthy, Kiran Chawla; Host and MTB genome encoded miRNA markers for diagnosis of tuberculosis; Tuberculosis; (116);2019, 37-43
Quartile 2; Impact factor – 2.369
Publication Link: https://www.sciencedirect.com/science/article/pii/S1472979218304979?via%3Dihub


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Dr Raghu Chandrashekar - Associate Professor, Dept. of Pharmaceutical Biotechnology, MCOPS, Manipal

Title of Seed grant project:: Inhibition of Herpes Simplex Viral Replication by Silencing the Expression of a Tegument Protein VP 1/2 for the Treatment of HSV infection
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Manda V, Josyula VR, Hariharapura RC. siRNA intervention inhibiting viral replication and delivery strategies for treating herpes simplex viral infection. VirusDis 2019
Quartile 3; Impact factor – 0.846
Publication Link: https://link.springer.com/article/10.1007%2Fs13337-018-00508-z


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